Biochem/physiol Actions

Reversible: yes

Cell permeable: yes

General description

A cell-permeable triazine derivative that is shown to increase cellular phosphorylation level of mTOR Ser2448 (20% and 42% above basal in 1 h, respectively, with 1 and 2 µM MHY1485) and downstream substrate 4E-BP Thr37/46 (29% and 34% above basal in 1 h, respectively, with 1 and 2 µM MHY1485) in rat liver Ac2F cells, affecting culture viability only at much higher concentrations (by 20% after 24 h 20 µM treatment). MHY1485 induced cellular LC3-II accumulation in Ac2F (117%, 150%, and 250% of vehicle control LC3-II/LC3-I ratio, respectively, with 0.5, 1, and 2 µM treatment for 6 h; 142% and 172% of control ratio, respectively, with 2 µM treatment for 1 h or 12 h) is reported to be a result of autophagy inhibition due to reduced fusion between autophagosomes and lysosomes as demonstrated by a reduction of starvation-induced AdGFP-LC3 and lysosome dye co-localization (27% and 32.7% above control co-localization in 6 h serum deprived cultures with or without 2 µM MHY1485; 21% above control co-localization with 2 µM MHY1485 treatment alone and without serum starvation). The mechanism of mTOR activation by MHY1485 is currently unknown.

A cell-permeable triazine derivative that is shown to increase cellular mTOR Ser2448 and downstream substrate 4E-BP Thr37/46 phosphorylation level in rat liver Ac2F cells (1 to 2 µM for 1 h), affecting culture viability only at much higher concentrations (by 20% after 24 h 20 µM treatment). MHY1485 induced cellular LC3-II accumulation in Ac2F is reported to be a result of autophagy inhibition due to reduced fusion between autophagosomes and lysosomes. The mechanism of mTOR activation by MHY1485 is currently unknown.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Choi, Y. J., et al. 2012. PLoS. One.7, e43418.

Packaging

Packaged under inert gas

10 mg in Glass bottle

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Warning

Toxicity: Standard Handling (A)